EVALUATION OF MELANOGENIC AND ANTI-VITILIGO ACTIVITIES OF HOMEOPATHIC PREPARATIONS ON MURINE B16F10 MELANOMA CELLS

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vitiligoABSTRACT
Background: Vitiligo is a multi-factorial disorder where the skin loses pigments. Homeopathic medicines are known to be effective for vitiligo. The melanogenic activity of homeopathy has not been evaluated in lab models.
Materials and Methods: Cell viability assay was performed to observe the cytotoxic effect of homeopathy preparations on melanoma cells. The effect of homeopathy preparations on the production of melanin in melanocytes was determined using B16F10 melanoma cells. The activity was checked at the end of 48 and 96 hours using 30c potency and 1:1 dilutions of homeopathy preparations. Result: Cell viability assay results showed no cytotoxic effect on the cells. Hydroquinone, Arsenicum sulphuratum flavum (ASF) and Phosphorous (30C) potency showed increase in melanin content as compared to cell and vehicle controls. However, statistically significant increase was seen with Hydroquinone and ASF. The diluted form (1:1) of all the three homeopathy preparations exhibited slight increase in the melanin content as compared to both the controls but was less as compared to cell control. At the end of 96 hours, ASF and Phosphorus exhibited a slight increase in melanin content as compared to potentized control. There was no inhibition of Tyrosinase activity seen during the assay with the homeopathy preparations. Discussion and Conclusion: The experiment has thrown important finding supporting the very fundamental homeopathy principle, by stimulating melanogenesis in the small (potentized) dose of the substance which in the crude form is known to be induce vitiligo.
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Rajesh Shah (born January 18, 1963) is a practicing homeopath in Mumbai, India. He was one of the first to start online medical practice in India in 1995. He has conducted clinical trials using conventional research methods for HIV and Hepatitis C, using the respective nosodes, and concluded that high dilution, potentised homeopathic preparation from HIV virus could increase CD4 cells and reduce viral load in the responder group. In the Homeopathic Pathogenetic Trial of the alkaloids Capsaicin and Dihydrocapsaicin, the interesting finding was that about 40% of the healthy volunteers produced symptoms pertaining to pain, with high-dilution, potentised medicine from the source materials which has known capacity to produce pain in crude form. He has revamped the method of preparation and introduced a new nosode sourced from Mycobacterium Tuberculosis. This study prepared a nosode at the Haffkine Institute, sourced from four strains of the organisms, including Multidrug resistance Tuberculosis. The nosode sourced from Hepatitis C virus produced anti-cancer effect on cell lines in a laboratory model.The research demonstrated that a nosode showed anti-cancer effect. By using advanced imaging techniques, research showed that the drug triggered the death of cancer cells through apoptosis, a process of controlled cell death, by decreasing two cancer related enzymes called telomerase and topoisomerase II in the liver cells. Rajesh Shah is Secretary of the Global Homeopathy Foundation, an NGO working for the promotion of homeopathy. He identified the need for a change in regulation in India in the Nosode category, responsible for lack of scientific research for new drug discovery. For getting the required regulation updated, and for creating awareness among the homeopaths, he initiated a petition to the Minister of AYUSH (Govt of India) for corrective measures. Preclinical evaluation of anti-arthritic activity of ultra-diluted preparations of capsaicin alkaloids (CP-10), tumor necrosis factor-α, and Magnesium phosphoricum was conducted on Wistar rats which concluded that ultra-diluted preparations exhibited definite anti-arthritic activity, demonstrated in his research at leading institution. In a prominent research, he and his team have demonstrated anti-malarial activity of selected homeopathic medicines in cell line model. Three homeopathic preparations including the very first homeopathic medicine China officinalis and one of the latest medicines Plasmodium falciparum nosode have shown disease modifying properties in an in-vitro model by inhibition of hemozoin (beta Hematin) formation. Hemozoin formation is a validated target for most known antimalarial drugs today. Another research has demonstrated stimulation of melanin with homeopathic medicines in cell line model, at leading BYL Nair Hospital’s department of Clinical Pharmacology, Mumbai.

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